In December 2022, Parties to the Convention on Biological Diversity adopted the landmark Kunming-Montreal Global Biodiversity Framework (GBF) and an associated package of decisions. These set out an ambitious plan to transform society’s relationship with nature and ensure that the vision of ‘living in harmony with nature’ is fulfilled by 2050. These decisions recognize the need for urgent and transformative action across the whole-of-society to halt and reverse biodiversity loss and to ensure successful implementation of the GBF. Businesses have a key role in supporting successful delivery of the GBF and associated decisions. This technical brief explores the key areas where business contributions can enhance implementation.