The importance of ecological connectivity for achieving biodiversity conservation outcomes is reflected within the draft goals and targets of the post-2020 global biodiversity framework. Ecological connectivity is a core element of draft Goal A which seeks to ensure “the integrity, connectivity, and resilience of natural ecosystems ….”. In addition, draft target 1 seeks to improve spatial planning to enhance ecological connectivity and deliver conservation outcomes; draft Target 2 applies restoration to enhance connectivity; draft Target 3 seeks to expand area-based conservation that is well-connected, and draft Target 12 seeks to increase connectivity of ‘green and blue spaces in urban areas. Given this recognition of the importance of ecological connectivity for achieving the 2050 Vision for Biodiversity, understanding how ecological connectivity might be measured is a key need for the post-2020 global biodiversity framework.